Journal: iScience

Article Title: Interferon-γ and IL-27 positively regulate type 1 regulatory T cell development during adaptive tolerance

doi: 10.1016/j.isci.2025.112308

Figure Lengend Snippet: Rapidly induced Il10 -expressing T cells are Tr1-like cells and have a transcriptionally delayed program (A) Re-analysis of GEO: GSE165817 (Elliot et al. ) where Tg4 Nr4a3 -Tocky Il10 -EGFP mice were administered with 0 (control), 8, or 80 μg [4Y]-MBP in 200 μL PBS s.c. and spleens harvested at 4, 12, and 24 h for bulk RNA isolation and transcriptome analysis. Heatmap of markers associated with early T cell activation, Th1 phenotype or Tr1-like cells arising after expression of Th1 cell hallmarks. (B) Gene set enrichment analysis (GSEA) using Tr1 genes defined in Chen et al. comparing 12 h gene expression in 80 μg vs. 0.8 μg immunized mice. (C) Heatmap of rlog-normalized data for GSEA core-enrichment genes for weak (0.8 μg) versus strong (80 μg) conditions. Tg4 Nr4a3 -Tocky Il10 -EGFP mice were administered 4 mg/kg [4Y]-MBP in 200 μL PBS s.c., and 24 h later CD4 + Il10- EGFP+ and CD4 + Il10 -EGFP − T cells were sorted for analysis by mRNA-seq. (D) Transcriptome analysis of CD4 + Nr4a3 -Timer + Il10 -EGFP ± as a heatmap of curated Tr1 differentially expressed genes (DEGs) between conditions at 24 h. (E–G) Compared temporal expression rlog transcripts from GEO: GSE165817 (Elliot et al. ) for Ifng against Il10 (E), Ifng against Lag3 (F), and Lag3 against Il10 (G) from Tg4 Nr4a3 -Timer Il10 -EGFP mice administered with 80 μg [4Y]-MBP in 200 μL PBS s.c. and mRNA transcripts depicted at 4, 12, and 24 h.

Article Snippet: GSEA 4.3.3 , UC San Diego and Broad Institute , https://www.gsea-msigdb.org/gsea/index.jsp.

Techniques: Expressing, Control, Isolation, Activation Assay, Gene Expression

Journal: iScience

Article Title: Interferon-γ and IL-27 positively regulate type 1 regulatory T cell development during adaptive tolerance

doi: 10.1016/j.isci.2025.112308

Figure Lengend Snippet:

Article Snippet: GSEA 4.3.3 , UC San Diego and Broad Institute , https://www.gsea-msigdb.org/gsea/index.jsp.

Techniques: Recombinant, Saline, Staining, Red Blood Cell Lysis, Sequencing, Software

Journal: eLife

Article Title: Ancestral acetylcholine receptor β-subunit forms homopentamers that prime before opening spontaneously

doi: 10.7554/eLife.76504

Figure Lengend Snippet:

Article Snippet: Single-channel detections were performed using the program TAC 4.3.3 (Bruxton).

Techniques: Modification, Recombinant, Construct, Sequencing, Mutagenesis, Software